By Method 827E: Semivolatile Organic Compounds by Gas Chromatography / Mass Spectrometry


A TIC (Total Ion Chromatogram) is a chromatogram created by summing up intensities of all mass spectral peaks belonging to the same scan. The TIC is compared to a GC chromatogram. Note that the TIC includes background noise as well as sample components.

This method is used to determine the concentration of semivolatile organic compounds in extracts prepared from many types of solid waste matrices, soils, air sampling media and water samples.

 This method can be used to quantitate most neutral, acidic, and basic organic compounds that are soluble in methylene chloride (or other suitable solvents provided that the desired performance data can be generated) and are capable of being eluted, without derivatization, as sharp peaks from a gas chromatographic fused-silica capillary column coated with a slightly polar silicone. Such compounds include PAHs, chlorinated hydrocarbons, chlorinated pesticides, phthalate esters, organophosphate esters, nitrosamines, halo ethers, aldehydes, ethers, ketones, anilines, pyridines, quinolines, aromatic nitro compounds, and phenols (including nitrophenols).

The samples are prepared for analysis by GC/MS using the appropriate sample preparation (refer to Method 3500) and, if necessary, sample cleanup procedures (refer to Method 3600). The semi volatile compounds are introduced into the GC/MS by injecting the sample extract into a GC equipped with a narrow-bore fused-silica capillary column. The GC column is temperature-programmed to separate the analytes, which are then detected with an MS connected to the GC. Analytes eluted from the capillary column are introduced into the MS via a direct connection. Identification of target analytes is accomplished by comparing their mass spectra and retention times (RT) with the mass spectra and RTs of known standards for the target compounds. Quantitation is accomplished by comparing the response of a major (quantitation) ion relative to an internal standard (IS) using an appropriate calibration curve for the intended application.

-SW-846 Update IVA

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